NWLSS™一氧化氮非酶检测试剂盒是一种用于测量生物样品中总硝酸盐和总亚硝酸盐的一氧化氮系统。该试剂盒最适合于蛋白质水平含量较高的更复杂的生物样本。注:此检测方法需要过夜孵育以有效降低样品硝酸盐含量。

Nitric Oxide (NO) is a biologically relevant free radical and cell signaling molecule. In normal cellular physiology it was originally characterized as an endothelial relaxation factor (EDRF) and is most commonly associated with its role in regulation of vasodilation. From a pathogenic standpoint the free radical nitric oxide (NO•) is known to combine with superoxide (O2•) to form peroxynitrite (ONOO-) which is a potent oxidant. Since ONOO– (NO3-) is essentially an unstable isomer of nitrate, it can react with tyrosine residues in proteins to create nitrotyrosine, a biomarker of oxidative mediated modification of proteins.

Nitric oxide degrades rapidly to nitrate (NO3) and nitrite (NO2) in aqueous biological systems. When NO3 is reduced to NO2 measurement of nitrite provides a simple, indirect means to quantify nitric oxide produced in a wide array of experimental model systems.

The test method is based on measurement of total sample nitrites using Griess Reagent. Since Griess reagent does not detect nitrate NO3, This kit employs metallic cadmium for reduction of nitrate to nitrite. The advantage of using cadmium instead of the enzyme Nitrate Reductase is that sample reduction is not affected by acidic reagents used to precipitate proteins prior to assay.

检测方法：比色法；

应用：量化生物样品中的一氧化氮 (NO)。通常是检测蛋白质含量较高的样品时的首选方法；

样品：血浆、细胞裂解液、组织匀浆；

Specificity：Nitrites or total nitrate & nitrite after nitrate reduction；

Sensitivity：(sens)；

Storage and Stability:9 months from date of manufacture when stored at 2-8C；

Kit Contents:

Microplate (96 well clear, low binding, flatbottom)

Nitrite Standard (500 µM NaNO₂)

ZnSO₄ (30% wt./vol.)

Nitrate Reductase (Lyophilized)

Reagent A: (Sulfanilamide (p-Aminobenzenesulfonamide) in 3N HCl)

Reagent B: (N-(1-Naphthyl) ethylenediamine dihydrochloride in deionized H₂O)

Granulated Cadmium (cadmium (Cd⁺⁺) beads)

Acid Wash Solution (0.1 M HCl)

Basic Wash Solution (0.1M NH₄OH)