胶原酶(Collagenase),即:胶原蛋白酶,又称:梭菌肽酶A,来源于溶组织梭菌(Clostridium histolyticum)。
CAS:9001-12-1,EINECS编号232-582-9;Enzyme Commission(EC) Number:3.4.24.3;MDL编号:MFCD00130830。
胶原酶I型(Collagenase Type I)用于上皮、肺,脂肪和肾上腺组织细胞的分离。
From………………Clostridium histolyticum
Appearance………………….tan to brown power;
Purity………………0.25-1.0 FALGPA units/mg solid, ≥125 CDU/mg solid;
Mol wt…………..68,000 - 125,000;
Optimum pH………..6.3-8.8;
Solubility………….TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 1-2 mg/mL at 37 °C。
Collagenase from Clostridium histolyticum has extensive use in biological studies, where it is used to prepare isolated cell suspensions.
This product is suitable for the disaggregation of human tumor, mouse kidney, human adult and fetal brain, lung and many other epithelia tissues. It has also been shown to be effective in liver and kidney perfusion studies, digestion of pancreas, isolation of nonparenchymal rat liver cells and hepatocyte preparation. Collagenase has also been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products. This enzyme has been tested for the release of heptatocytes at a concentration of approximately 1 mg/mL. Concentrations for digestion range from 0.1 to 5 mg/mL.
Storage:−20°C.