P450去甲基化荧光活性试剂盒
The DetectX® P450 Fluorescent Activity Kit allows activity measurement of any demethylating P450 system WITHOUT additions to the P450:Substrate reaction by measuring the formaldehyde generated by demethylation. Lab-to-lab comparisons are possible with inclusion of a formaldehyde reference standard. 

检测类型:活性检测

样本类型:Any P450 system or biological system producing demethylation product, Formaldehyde

检测时间:45分钟

样品数/板:41 in Duplicate

DetectX^® P450荧光活性检测试剂盒用于检测任何去甲基P450系统的活性，而无需添加任何P450:底物反应。该测定法检测由去甲基化产生的甲醛，并在P450反应终止后读取信号。30分钟荧光底物孵育和510 nm检测读数的便捷平板分析。在3A4、2D6和2B4 P450系统中用红霉素，右美沙芬或苯丙胺进行了测试。在进行操作前请阅读完整试剂盒说明书或咨询中昊新生(zhscience.com)。

The P450 Fluorescent Activity kit is designed to quantitatively measure the enzymatic activity of formaldehyde-producing Cytochrome P450s. No separation or washing is required. The kit has been validated for several P450 systems. This kit should work with any biological system that produce formaldehyde as a product of demethylation. Please read the complete kit insert before performing this assay

背景:
细胞色素P450s (P450s)是含血红素酶的超家族，在底物特异性和催化活性方面显示出极大的多样性。P450s在其反应中使用过多的外源和内源化合物作为底物。通常它们是多组分电子转移反应的一部分。真核P450酶的催化涉及一个多步反应循环，该循环包括两个步骤，其中从氧化还原伴侣完成电子转移。二黄素蛋白，NADPH细胞色素P450还原酶同时包含FAD和FMN，可以转移催化循环所需的两个电子。在一些P450反应中，反应周期的第二个电子也可以由细胞色素b5传递。哺乳动物药物代谢系统的P450酶和辅助因子嵌入内质网的膜中。由于药物相互作用通常会抑制细胞色素P450的活性，因此P450在新药物实体的开发中起着至关重要的作用。

Cytochrome P450 enzymes, along with cofactors, function to metabolize drugs and play a crucial role in the development of new drug entities, as drug interactions commonly inhibit enzyme activities. The enzymes are a superfamily of heme containing enzymes. They display tremendous diversity regarding substrate specificity and catalytic activity by using both exogenous and endogenous compounds as substrates in their reactions and forming part of multicomponent electron transfer reactions.

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