The DetectX® Human Osteopontin ELISA Kit is designed to quantitatively measure human Osteopontin present in biological samples and tissue culture media, and includes a reference standard.

检测类型: Sandwich ELISA
样本类型: EDTA Plasma, Urine, Milk, Tissue Culture Media
灵敏度: 0.246 ng/mL
适用物种: 人
检测时间: 2.5小时
样品数/板: 40 in Duplicate
读数: Colorimetric, 650 nm and 450 nm

检测原理:
The Osteopontin Human (OPN) ELISA Kit quantitatively measures human Osteopontin levels in EDTA plasma, urine, milk, and tissue culture media. The Osteopontin Human (OPN) ELISA Kit is a sandwich ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.

Use our provided Osteopontin standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our mouse anti-human Osteopontin antibody and incubate at room temperature for 1 hour. Add the peroxidase-conjugated human Osteopontin monoclonal antibody. Then incubate the mixture covered at room temperature for 1 hour. The immunological reaction occurs between the peroxidase-conjugated human Osteopontin monoclonal antibody and the Osteopontin antigen in the sample or standard.

After the 1-hour incubation, wash away the excess peroxidase-conjugated Osteopontin monoclonal antibody and add the TMB substrate. The TMB substrate reacts with the bound peroxidase-conjugated Osteopontin monoclonal antibody generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the Osteopontin concentration in the samples.

背景:
Osteopontin (OPN) is vital in mineralization, blood vessel formation, cell survival, inflammation, and inhibition of iNOS expression, which protects tumor cells from NO-mediated macrophage cytotoxic attack. It is also a key molecule in neoplastic transformation and cancer development in various tumors, playing a critical role in invasiveness, progression, and metastasis. Plasma OPN is a positive indicator of colon and lung cancers and metastatic carcinomas. In addition, OPN mRNA expression increases approximately 40-fold in infarct tissue after myocardial infarction.