NWLSS™ Urinary 8-Isoprostane (15-Isoprostane F2t) ELISA Kit

尿液8-异前列腺素（15-异前列腺素F2t） ELISA试剂盒，该试剂盒用于尿液样本中游离8-异前列腺素(15-异前列腺素F2t)的定量分析，这是表征最好的异前列腺素。

如果要检测血浆、细胞和组织匀浆中的8-异前列腺素(15-异前列腺素F2t)请选择我们的NWLSS™ 8-异前列腺素[NWK-ISO01]试剂盒。

NWLSS™尿液8-异前列腺素(15-异前列腺素F2t)的优点：

◆检测低至50 pg /mL

◆对8-异前列腺素(15-异前列腺素F2t)具有高度特异性: 对9α,11β-Prostaglandin F2α, Prostaglandin F2α, 6-Keto-Prostaglandin F1α, Prostaglandin E2, Prostaglandin D2或Arachidonic Acid检测到低或未检测到交叉反应性

◆可以简单、快速地对来自任何物种的尿液样品中的8-异前列腺素(15-异前列腺素F2t)进行定量，而无需进行困难且耗时的提取

Isoprostanes are prostaglandin-like compounds that are produced by free radical mediated peroxidation of lipoproteins. This kit is for the quantification of 8-Isoprostane (15-isoprostane F2t), also known as 8-epi-PGF2aor 8-iso-PGF2a in urine samples. Levels of 8-Isoprostane (15-isoprostane F2t) in urine are useful for the non-invasive assessment of oxidant stress in vivo. 8-Isoprostane (15-isoprostane F2t) has also been shown to be a potent vasoconstrictor in rat kidneys and rabbit lungs, and may play a causative role in atherogenesis. Elevated isoprostane levels are associated with hepatorenal syndrome, rheumatoid arthritis, atherosclerosis, and carcinogenesis. This kit can be used for the quantification of free 8-Isoprostane (15-isoprostane F2t) in urine serum samples without the need for prior purification or extraction.

Test Principle

This assay utilizes a competitive enzyme-linked immunoassay (ELISA) strategy. Prior to assaying, all samples must be treated with an Enhancing Reagent. After treatment with Enhancing Reagent, 8-Isoprostane (15-isoprostane F2t) in the samples or standards is allowed to compete with 8-Isoprostane (15-isoprostane F2t) conjugated to horseradish peroxidase (HRP) for binding to a polyclonal antibody specific for 8-Isoprostane (15-isoprostane F2t) coated on a microplate. Later TMB substrate addition results in a blue color development that is inversely proportional to the quantity of 8-Isoprostane (15-isoprostane F2t) in the original samples or standards. Addition of an acid stop solution causes a color change to yellow where absorbance is read at 450 nM.

Format: 96 Well Competitive ELISA

Sample Requirements:Urine from any species

Specificity:

(100.0%) 8-Isoprostane (15-isoprostane F2t)

(4.1%) 9α,11β-Prostaglandin F2α

(3.0%) 13,14-Dihydro-15-Keto-PGF2α

(<0.01%) 9α,11β-Prostaglandin F2α

(<0.01%) Prostaglandin F2α

(<0.01%) 6-Keto-Prostaglandin F1α

(<0.01%) Prostaglandin E2

(<0.01%) Prostaglandin D2

(<0.01%) Arachidonic Acid

Sensitivity:0.05 ng/mL

Effective Range:.05 ng/mL - 10 ng/mL

Storage and Stability:6 months from the date of manufacture

Kit Contents:

96-well microtiter plate, precoated with Anti 8-Isoprostane (15-isoprostane F2t)

8-Isoprostane (15-isoprostane F2t) Standard

Enhancer Reagent

5X Wash Buffer

5X Dilution Buffer

TMB Substrate

8-Isoprostane (15-isoprostane F2t) HRP Conjugate

Reagent Trough for multi-channel pipettor