NWLSS™ 硝基酪氨酸ELISA试剂盒推荐用于检测血浆、血清和组织匀浆样品中的亚硝基化蛋白。过氧硝酸盐相关研究的优秀工具，被推荐作为蛋白相关氧化/硝化应激相关增强的生物标志物。该硝基酪氨酸ELISA试剂盒是一种简单的夹心法ELISA。

Nitrate (NO3) and nitrite (NO2) production are typically used as markers of nitric oxide production (NO) production. These analytes by themselves fail to address the eventual fate of NO or the possible adverse effects associated with its excess production and reaction with free radical species in vivo. Active NO metabolites can react with superoxide to form peroxynitrite (ONOO-) a powerful oxidant and nitrating agent. Subsequent reaction of peroxynitrite with proteins results in nitrotyrosine formation. As a stable end product of peroxynitrite mediated oxidation/nitration, Nitrotyrosine can be used as a surrogate index of NO dependent damage in vivo and has been associated with multiple disease states.

Test Principle

The NWLSS™ Nitrotyrosine ELISA test is a simple "sandwich" ELISA using a plate bound capture antibody (anti nitrated KLH) to nitrotyrosine and a biotinylated secondary tracer antibody. Addition of streptavidin-peroxidase followed by tetramethylbenzidine (TMB) facilitates color development directly proportional to the nitrotyrosine present in the sample. The reaction is stopped using a citric acid solution and the assay is read on a plate reader at 450 nm.

格式：192孔可拆酶标板（2块板）

样品要求：血浆、血清等生物样品

特异性：亚硝基化蛋白加合物

灵敏度：2 nM

储存和稳定性：在指定温度下储存，自生产日期起1年

Kit Contents:

Precoted Microwell strips and strip frames

Wash Buffer

Dilution Buffer

Nitrotyrosine Standard

Biotinylated anti nitrotyrosine tracer

Streptavidin Peroxidase

TMB Substrate

Stop Solution