ABSbio™人TNF-α ELISA试剂盒用于定量检测天然或重组人TNF-α。该试剂盒提供的试剂足以检测96孔ELISA板的人TNF-α样品。

人TNF-α ELISA试剂盒是一种快速、灵敏、可靠的检测人TNF-α方法，适用样品：血清、血浆和细胞培养基，检测范围：8-2000 pg/ml。 

Tumor necrosis factor-alpha (TNF-α, or TNF) is secreted by macrophages in response to inflammation, infection and cancer.

ABSbio™ Human TNF-α ELISA Kit provide a rapid, sensitive and reliable assay for the determination of Human TNF-α in serum, plasma & cell culture media. The strip plate is coated with the antibody and pre-blocked to provide timesaving for high-throughput users. Human TNF-α containing samples or standards and a biotinylated detection antibody directed against Human TNF-α are added subsequently and then followed by washing step. Streptavidin-HRP was added and unbound conjugates were washed away. Finally, TMB was used to visualize HRP enzyme reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TNF-α in samples.

The kit is designed to quantitatively measure natural and/or recombinant Human TNF-α within the range of 8–2000 pg/ml. The kit is sufficient to assay human TNF-α in approximately 96 ELISA plate wells.

别名：DIF; TNF-A; TNFa; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2; Tumor Necrosis Factor Alpha; 肿瘤坏死因子α