人单核细胞趋化蛋白1 ELISA试剂盒

人MCP-1 ELISA试剂盒旨在以夹心ELISA形式定量检测天然或重组人MCP-1，检测范围为15–2500 pg/ml，适用样品：血清，血浆及细胞培养基。

Monocyte chemoattractant protein-1(MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF.

ABSbio™ Human MCP-1 ELISA Kit provide a rapid, sensitive and reliable assay for the determination of Human MCP-1 in serum, plasma & cell culture media. The strip plate is coated with the antibody and pre-blocked to provide timesaving for high-throughput users. Human MCP-1 containing samples or standards and a biotinylated detection antibody directed against Human MCP1 are added subsequently and then followed by washing step. Streptavidin-HRP was added and unbound conjugates were washed away. Finally, TMB was used to visualize HRP enzyme reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MCP-1 in samples.

The kit is designed to quantitatively measure natural and/or recombinant Human MCP-1 within the range of 15–2500 pg/ml. The kit is sufficient to assay human MCP-1 in approximately 96 ELISA plate wells.