ABSbio™大鼠IL-6 ELISA试剂盒/大鼠白介素6 ELISA试剂盒,用于以夹心ELISA形式定量检测天然或重组大鼠IL-6,检测范围为23-2000 pg/ml,适用样品:血清,血浆及细胞培养基。

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.

ABSbio™ Rat IL-6 ELISA Kit provide a rapid, sensitive and reliable assay for the determination of Rat IL-6 in serum, plasma & cell culture media. The strip plate is coated with the antibody and pre-blocked to provide timesaving for high-throughput users. Rat IL-6 containing samples or standards and a biotinylated detection antibody directed against Rat IL-6 are added subsequently and then followed by washing step. Streptavidin-HRP was added and unbound conjugates were washed away. Finally, TMB was used to visualize HRP enzyme reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat IL-6 in samples. The kit is designed to quantitatively measure natural and/or recombinant Rat IL-6 within the range of 23–2000 pg/ml. The kit is sufficient to assay Rat IL-6 in approximately 96 ELISA plate wells.