ABSbio™牛EGF ELISA试剂盒/牛表皮生长因子ELISA试剂盒,旨在以夹心ELISA形式定量检测样品中的牛EGF含量,检测范围6–400 pg/ml。
This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for bovine EGF has been pre-coated onto a microplate.
Standards and samples are pipetted into the wells and any EGF present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for bovine EGF is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of EGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Storage:
Store at 4 °C. The kit can be used in 3 months.
400-888-0207
