RNeasy® PowerMicrobiome® Kit用于从粪便和肠道物质中分离总RNA。

For the isolation of total RNA from stool and gut material

The RNeasy PowerMicrobiome Kit is designed for fast and easy purification of total RNA from samples high in PCR inhibitors, including stool, gut material, dried feces, contaminated buccal swabs and secretions. Inhibitor Removal Technology® (IRT) ensures complete removal of the inhibitory substances often contained in these materials (e.g., undigested plant material in the gut or heme compounds from lysed red blood cells, abundant in stool). The result is RNA that is ready to use in the most demanding downstream applications. Genomic DNA is removed on column by using the provided DNase I and reaction buffer. RNA is eluted in RNase-free water and is ready for downstream applications.

Principle and procedure
Stool or biosolid samples are processed through a lysis step that uses bead beating and a strong chemical lysis buffer for efficient extraction of tough microorganisms. We recommend starting with 0.25 grams of material.

In the next step, the binding solution (PM3) holds the total nucleic acids in the lysate. DNA is removed using on-column DNase followed by a wash solution. This removes the enzyme and any digested nucleic acids. The RNA eluted in RNase-free water is ready to use in any downstream application.