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Anhydrotrypsin
货号:ANHT | 规格:1.0 mg | 价格:¥0.00 | 品牌:Molecular Innovations
Anhydrotrypsin是由北京中昊新生科技有限公司提供,Molecular Innovations专注于凝血和溶栓试剂,包括PAI-14、tPA、尿激酶、凝血因子、玻连蛋白、纤维蛋白原、纤连蛋白、肾素原/肾素、前激肽释放酶、激肽释放酶、白蛋白、纤溶酶原、纤溶酶、抗纤溶酶、凝血酶、抗凝血酶、补体成分和免疫球蛋白。
Treatment of trypsin with phenylmethylsulfonyl fluoride (PMSF) followed by a strong base leads to the conversion of Serine 195 to dehydroalanine at the active site (1). Alpha and beta forms of anhydrotrypsin are separated by immobilized soybean trypsin inhibitor. The activity of anhydrotrypsin is greatly reduced compared to native trypsin as quantified by chromogenic substrate (2). Anhydrotrypsin provided by our company is the single chain beta-form prepared from Type XIII bovine trypsin.
References
1. Ako, H. et al. (1972) Biochem Biophys Res Commun. 47:1402-1407.
2. Olson, S.T. et al. (1995) J. Biol. Chem. 270:30007-30017.
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