Human PAI-1 NBD labeled at the scissile bond of the reactive loop

Human PAI-1 NBD labeled at the scissile bond of the reactive loo

货号：HPAI-P1NBD | 规格：1.0 mg | 价格：￥0.00 | 品牌：Molecular Innovations

Human PAI-1 NBD labeled at the scissile bond of the reactive loop是由北京中昊新生科技有限公司提供，Molecular Innovations专注于凝血和溶栓试剂，包括PAI-888、tPA、尿激酶、凝血因子、玻连蛋白、纤维蛋白原、纤连蛋白、肾素原/肾素、前激肽释放酶、激肽释放酶、白蛋白、纤溶酶原、纤溶酶、抗纤溶酶、凝血酶、抗凝血酶、补体成分和免疫球蛋白。

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P1'-NBD PAI-1 was created by mutagenesis of the methionine residue (Met347) at the P1-P1' scissile bond to cysteine. This provides a free thiol group for labeling with NBD, a fluorescent probe highly sensitive to changes in solvation and hydrophobic environment. The fluorescence emission of P1'-NBD PAI-1 is quenched upon cleavage of the reactive center loop by a target proteinase.

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