ACTH (Adrenocorticotropic Hormone) Antibody   促肾上腺皮质激素抗体

The antibody produces a maximum biotin-streptavidin/HRP staining at a 1/500 – 1/1000 dilution in rat anterior/intermediate pituitary. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The recommened dilution series is 1/500-1/1000 in PBS/0.3% Triton X-100 – Bn-SA/HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg/mL of ACTH.

照片描述: IHC image of neurons staining for ACTH in the rat anterior pituitary gland. The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:1000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

宿主: Rabbit

规格/体积: 100 µL

性状: Lyophilized Whole Serum

反应性: Dove, Eel, Goldfish, Hamster, Human, Monkey, Mouse, Ram (Sheep), Rat, Trout

别名:  anti-ACTH

RRID:  AB_572216

免疫原: Adrenocorticotropic Hormone

Gene Symbol:  POMC