NRicher™ Ig

Enrichment of all isotypes and subclasses of Immunoglobulins

NRicher™ Ig用于免疫球蛋白所有isotypes和subclasses的富集，Biotech Support Group提供两个规格NRicher™ Ig，50 preps（NIMM-50）和10 preps（NIMM-10）。

显著特点

· 消耗性的化学衍生磁珠，与物种无关，因为它们不是来自抗体

· 从动物和人的血清或血浆中富集循环免疫复合物（Enrich circulating immune complexes），白蛋白去除率>90%

· 不需要任何专门的仪器，只需要一个微量离心机

· 磁珠格式适合自动化兼容（请咨询）

· 用于LC-MS分析的On-Bead消化，或用于任何功能性、酶促或免疫测定分析的选择性洗脱

A comprehensive analysis of the humoral immune response (the immunome) has potential to greatly impact research across numerous fields. For example, serum autoantibodies against tumor-associated antigens have recently emerged as early stage biomarkers for different types of cancers. Most autoantibody profiling work has been based on the reactivity of unbound antibodies towards antigens produced by a variety of strategies (i.e., cDNA libraries, phage display).

An alternative approach is based on the identification of Ig-bound antigens using Liquid Chromatography coupled to Mass Spectrometry (LC-MS). Such determination of antigens complexed with antibodies at a proteome scale is critical to understanding adaptive responses in the context of infection, autoimmunity, and cancer.

Human serum immunoglobulins comprise several classes IgG, IgA, IgM, IgD & IgE. IgG is the predominant human immunoglobulin class in plasma and comprises four subclasses; ~60% are IgG1, followed by ~30% IgG2, ~7% IgG3 and ~3% IgG4. To date, most of the circulating antibody complex research has been focused on IgG as the efficiency of recovering a representative pool of IgG antibodies is well established. Generally for human serum/plasma, Protein A binds with high affinity to IgG1, IgG2, and IgG4, but poorly to IgG3. Among the four IgG subtypes in mice, Protein A has the weakest affinity for IgG1 while Protein G has affinity for all four IgG subclasses. Neither Protein A or G bind particularly well towards IgA, IgM, IgD or IgE.

Nevertheless, the ability to enrich circulating immune complexes from sera or plasma from both animals and humans with high yield and without selective loss of isotypes or subclasses can provide more comprehensive profiles. NRicher™ Ig can provide such enrichment for all immunome profiling methods. For antigen reactivity profiling, elution conditions are mild (pH 9-10), and preserve functionality. For antigen identification, bound proteins can be digested on-bead, with seamless integration to LC-MS analysis.