For the isolation of microbial genomic DNA from all soil types

Features

•Efficient lysis of bacteria and fungi in all soil types
•Up to 8-fold higher DNA yields compared to alternative methods
•Recovery of inhibitor-free DNA for direct use in NGS applications
•Unbiased results accurately represent sample alpha diversity
•Automation on the QIAcube

Product Details

Extracting microbial DNA from soil samples can be challenging. QIAGEN’s new DNeasy PowerSoil Pro Kit is even more
effective than our original PowerSoil technology at isolating high yields of pure microbial DNA from all soil types,
including compost, clay and top soil. The kit features a novel bead tube and optimized chemistry for more efficient
lysis of soil bacteria and fungi. The kit also contains streamlined Inhibitor Removal Technology (IRT) to eliminate
the challenging inhibitors commonly found in soil and environmental samples in even less time. Sequencing results
reveal higher alpha diversity as measured by observed operational taxonomic units (OTUs) compared to other tested
methods. Extraction of DNA using the DNeasy PowerSoil Pro Kit can be automated on the QIAcube Connect.
Want to try this solution for the first time? Request a quote for a trial kit.

Performance

Significantly increased DNA yields from challenging samples

The DNeasy PowerSoil Pro Kit includes a novel bead tube and improved lysis chemistry, which enables isolation of up to
8-fold higher yields of DNA compared to the first generation DNeasy PowerSoil Kit and those of competitors in all soil
types tested . Both the DNeasy PowerSoil Kit and the new DNeasy PowerSoil
Pro Kit isolated high-quality DNA from challenging soil types.

Unbiased identification of total diversity and community representation in soil samples
DNA isolated using the DNeasy PowerSoil Pro Kit identified more bacteria (OTUs) in soil samples when compared with the
DNeasy PowerSoil Kit and those of two competitors 。

Principle

Inhibitor Removal Technology increases purity of isolated DNA

 The DNeasy PowerSoil Pro Kit features a streamlined Inhibitor Removal Technology (IRT) to decrease sample processing time.
In a comparison of methods for DNA isolation from soil, the DNeasy PowerSoil Pro Kit was the only method showing 260/280
 ratios near 1.8 for all soil types, as well as the highest 260/230 ratios, indicating the absence of inhibitors. Samples
isolated using the kit also showed no inhibition in PCR, in contrast to competitor methods 。

Procedure

The soil or stool samples are lysed via chemical and mechanical homogenization. Lysis buffer is added to a mixed zirconium
bead tube containing the sample. Bead beating can be carried out using a standard benchtop vortex with bead tube adapter or
the high-powered PowerLyser. Crude lysate is then subjected to inhibitor removal for cleanup. Following the cleanup, purified
lysate is mixed with an equal volume of DNA binding solution and passed through a silica spin filter membrane. The membrane is
washed with a two-step washing regime. Silica-bound DNA is then eluted using a 10 mM Tris elution buffer. Relevant downstream
applications for the isolated DNA include PCR, NGS and enzymatic digestion assays.