统一服务热线:400-888-0207 在线咨询 | 微信联系
首页 > 产品 > 产品类型 > 试剂 > 细胞检测
TC Protector MINI
货号:TCP-002 | 规格:10 mLX10 | 价格:¥0.00 | 品牌:KAC
TC Protector MINI/细胞冻存液是一种细胞冻存保护剂,不含动物源性成分或蛋白质,不粘稠,不形成气泡,用于需要添加血清的细胞,无血清细胞和无蛋白质细胞。
北京中昊生物是日本KAC中国的代理商,我们很荣幸为研究人员提供KAC产品的订购以及帮您解决产品的所有技术问题。

TC Protector是一种细胞冷冻保护剂(Cell Cryoprotective agent),不含任何动物源性成分或蛋白质,是化学定义的。可用于需要添加血清的细胞、无血清的细胞和无蛋白质的细胞。

TC Protector适用于细胞系细胞和正常细胞,以及ES细胞、成体干细胞和人iPS细胞。不粘稠,也不会形成气泡。因此,具有极好的可用性。


特征

根本不含动物源性成分,是化学定义的。

不含任何血清或蛋白质。

在冰箱中储存,取出后可立即使用。

粘性很小,可用性极好。

不需要特殊设备,例如程序冷冻机。可以直接存储于−80ºC。


冷冻方法

1. In case of adhesive cells, media should be changed at 80% confluence and be frozen on the next day.

In case of floating cells, media change (or media supplementation) is conducted and should be frozen on the next day.

*In order to freeze cells in a good condition, it is important to use cells from the logarithmic growth phase.

*Cells that have reached confluence or overgrown result in a decreased survival rate after freezing.

2. Cells are harvested according to the standard procedure and are centrifuged at 1,500 rpm for 1 minute.

3. It is diluted with TC Protector to achieve 5×105~1×107 cell/mL.

*Cell suspension is maintained in ice water when cell counts are measured.

*Since the appropriate cell number varies depending on cells, a pilot study must be conducted in advance. In general, 1×106 cells/mL is the target cell count.

4. Cells are stored at −80ºC in a Deep Freezer and are transferred into liquid nitrogen on the next day.


解冻方法

Ampules are placed in a warm water bath at 37ºC, and it is quickly thawed until it forms small ice mass.

Cell suspension is collected into a spitz tube and about 10× media is added. Then, it is centrifuged at 1,000–1,500 rpm for 1–2 minutes.

Cell pellet is checked and supernatant is discarded. Cells are seeded in accordance with the standard method.

相关产品
相关资料