Immunostar VAT抗体
VAT (Vesicular Acetylcholine Transporter) Antibody 囊泡乙酰胆碱通道抗体
The antibody produces strong labeling of VAChT at a dilution of 1/3,000 – 1/5,000 using biotin-streptavidin/HRP technique in rat basal forebrain. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.
Immunolabeling is completely abolished by pre-adsorption with synthetic rat VAChT (511-530). Immunolabeling of transfected cells demonstrates no cross reactivity with vesicular monoamine transporters.
照片描述: IHC image of neurons in the rat basal forebrain staining for VAT. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% rabbit serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, rabbit anti-goat secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
宿主: Goat
规格/体积: 100 µL
性状: Lyophilized Whole Serum
Species Reactivity: Human, Mouse, Rat
别名: Solute carrier family 18, member 3; solute carrier family 18 (vesicular monoamine) member 3; solute carrier family 18 (vesicular acetylcholine), member 3; rVAT; VACht, anti-VAT, anti-VACht
Gene Symbol: Slc18a3
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