2021-01-05
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优点 Advantages
无安全隐患 克隆效率高 无DNA损伤 减少漂白 穿过墙壁
我们不喜欢紫外线照射... 您的质粒将感谢您！ 无突变荧光 持续发光的荧光团 塑料不会妨碍我们
Is UV causing problems?
Decreased efficiency – UV transilluminators typically output light around 300 nm – well removed from the absorption maxima of most common dyes. Occasional Sunburn? – Many users of UV transilluminators have experienced, at one time or another, either a mild case of sun-burn or `spots before the eyes` as a result of spending too long either examining a gel or cutting out bands. The potentially harmful effects of shorter wavelength light (less than 400 nm) are well documented and were even the subject of a report from the Council on Scientific Affairs of the American Medical Association . | No UV output = No DNA damage DNA undergoes a number of reactions when exposed to UV light. Because the Dark Reader does not emit any UV light, the extent of DNA damage is drastically reduced compared to the damage produced by a UV device.
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| 100 ng of supercoiled (sc) plasmid was placed on either a Dark Reader (DR) or a 312 nm UV transilluminator (UV) for various times. The DNA was then digested with T4 endonuclease V, which excises T:T dimers, and run on an agarose gel and viewed. It is clear that as little as a 5 sec exposure to UV light is sufficient to convert almost 100% of the plasmid into the relaxed (rx) form and after 300 sec the DNA is completely fragmented. In contrast, a 300 sec exposure on the Dark Reader resulted in no detectable damage to the plasmid. | |
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