NWLSS™ Glutathione Peroxidase (GPx) Activity Assay Kit

一种基于速率的动力学检测试剂盒，用于定量包括血液制品、组织和培养细胞在内的各种样品中的谷胱甘肽过氧化物酶 (GPx)。 该测定适用于任何物种。

Glutathione Peroxidase (GPx) (EC 1.11.1.9) enzymes belong to a family of selenoproteins whose function is to catalyze the reduction of various peroxides. Four types of GPx (GPx-1 to 4) have been identified in mammalian species. GPx-1 is the classic intracellular form while GPx-2 to 4 are gastrointestinal, plasma and phospholipid hydroperoxide metabolizing forms respectively.

This assay is an adaptation of the method of Paglia and Valentine. Glutathione peroxidase (GPX) catalyzes the reduction of an organic peroxide (ROOH), oxidizing reduced glutathione (GSH) to form disulfde glutathione (GSSG). The oxidized glutathione is then reduced by glutathione reductase (GR) and b-nicotinamide adenine dinucleotide phosphate (NADPH) forming NADP+ (resulting in decreased absorbance at 340 nm) and recycling the GSH. Because glutathione peroxidase (GPx) is limiting, the decrease in absorbance at 340 nm is directly proportional to the glutthione (GPx) concentration.

格式：192 tests（2 × 96孔酶标板）。也可以在比色皿中进行检测，通过分光光度计进行测量，每个试剂盒可进行100次测试

样品要求：组织和细胞裂解物

特异性：谷胱甘肽过氧化物酶

灵敏度：LLD = 5.0 mU/mL（通过读板器/酶标仪） LLD = 1.0 mU/mL（通过分光光度计）

预期用途：GPx活性的定量测量

储存和稳定性：在2-8℃储存时，自制造之日起12个月

Kit Contents:

NADPH Reagent (containing GSH)

NADPH Diluent

H2O2 Reagent

Assay Buffer

96 Well Microplates