Protein Concentration: 0.20 mg/ml (vortex before apply for gel electrophoresis)
Size/Physical State: 100 μl (20 applications of 5 μl each). ApoAs/Cs Standard is a ready-to-use format - no mixing, heating or reducing required. It is pre-reduced and contains reducing reagents in the loading buffer. The protein standards resolve into bands with 6.6 kD for apoCI, 8.5 kD for apoCII, and 8.7 kD for ApoCIII, and 28 kD for Apo AI; ApoAII (8.7 kD) appears between ApoCI and ApoCII.
Expiration: One year from the date of purchasing
Source: From purified ApoAI/AII and ApoCs; fresh human plasma that has tested negative for Hepatitis C, HIV-I and HIV-II antibodies as well as Hepatitis surface antigens.
Use: The Un-Stained ApoAs/Cs Standard allows you to check the result of your gel run and to judge western transfer efficiency. The attached gel was running with 4 µl of each for lane 1 (ApoAs/Cs) and lane 2 (Std Mix2) on an Invitrogen 18% SDS-Gel.
Buffer: In loading buffer consists of Tris-HCl, MeSH, SDS, glycerol, and bromophenol.
Storage: -20ºC for short and long-term storage. Aliquot to avoid repeated freezing and thawing.