产品性状: 0.5 ml冻干粉;
抗体浓度: 1 mg/ml;
来源: 山羊抗HNE多克隆抗体通过4 HNE修饰的Protein-Sepharose™亲和柱纯化,并用生物素标记;
保存状态: 75 mM PBS, 75 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH 7.2;
稳定剂:10 mg/ml牛血清白蛋白;
特异性: 特异性结合到4 HNE修饰的低密度脂蛋白(LDL)和其他4 HNE修饰的蛋白。稀释后用于免疫印迹和ELISA,稀释范围:200-40000;
应用: 该抗体可用于检测血浆和其他4-HNE修饰蛋白的4 HNE修饰蛋白;免疫测定和免疫印迹。
重构和存储:Freeze-dried product should be stored refrigerated until opened. After opening, restore to 0.5 ml with distilled water. If it is not completely clear after standing for 1-2 Hours at room temperature, centrifuge the product. It is stable for several weeks at 4ºC as an undiluted liquid. Do not use for more than one day after dilution. For extended storage after reconstitution, we suggest aliquot to avoid repeated freezing and thawing; or the addition of an equal volume of glycerol to make a final glycerol concentration of 50%, followed by storage at - 20ºC. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol.