NWLSS™ 一氧化氮酶促（比色法）检测试剂盒是一种用于测量生物样品中的总硝酸盐和总亚硝酸盐形式的一氧化氮的系统。

Nitric Oxide (NO) is a biologically relevant free radical and cell signaling molecule. In normal cellular physiology it was originally characterized as an endothelial relaxation factor (EDRF) and is most commonly associated with its role in regulation of vasodilation. From a pathogenic standpoint the free radical nitric oxide (NO•) is known to combine with superoxide (O2•) to form peroxynitrite (ONOO-) which is a potent oxidant. Since ONOO– (NO3-) is essentially an unstable isomer of nitrate, it can react with tyrosine residues in proteins to create nitrotyrosine, a biomarker of oxidative mediated modification of proteins.

Nitric oxide degrades rapidly to nitrate (NO3) and nitrite (NO2) in aqueous biological systems. When NO3 is reduced to NO2 measurement of nitrite provides a simple, indirect means to quantify nitric oxide produced in a wide array of experimental model systems.

Test Principle

The test method is based on measurement of total sample nitrites using Griess Reagent. Since Griess reagent does not detect nitrate NO3, this kit employs nitrate reductase for reduction of nitrate to nitrite (Fig. 1). Using purified plant nitrate reductase with NADH helps prevent NADPH interference with the Griess reaction as has been previously reported.

方法：比色法；

应用：量化生物样品中的一氧化氮 (NO)；

样品要求：血浆、细胞裂解液、组织匀浆；

特异性：硝酸盐还原后的亚硝酸盐或总硝酸盐和亚硝酸盐；

灵敏度：测定中为1 pmol/mL或1µM；

储存和稳定性：2-8℃储存。

试剂盒内容：

微孔板（96 孔透明，低结合，平底）

MOPS 缓冲液（样品稀释缓冲液）

硝酸盐标准品（500 µM KNO3）

硝酸盐还原酶（冻干）

硝酸还原酶缓冲液

NADH（冻干）

试剂 A：（磺胺（对氨基苯磺酰胺）的 3N HCl 溶液） )

试剂 B：（去离子水中的 N-(1-萘基)乙二胺二盐酸盐）