NWLSS™ Human Macrophage Inhibitory Cytokine (MIC-1) ELISA Kit

人巨噬细胞抑制因子(MIC-1) ELISA试剂盒用于定量检测血清、血浆、组织和细胞裂解液及其他可能存在MIC-1的生物液体中的MIC-1。

Macrophage inhibitory cytokine (MIC-1) is a divergent member of the TGF-β superfamily. MIC-1 is distributed in various tissues, being highly expressed in macrophages, choroid plexus, prostate, lung, kidney proximal tubules, placenta and intestinal mucosa. It is poorly expressed in the heart although it has been described as a prognostic marker in acute myocardial infarction as well as an independent predictor of chronic heart disease mortality. Initially, MIC-1 was considered to function primarily as a macrophage inhibitor, but recent studies suggest that it is pleiotropic regulating a myriad of cellular processes such as the cell cycle, proliferation, differentiation, and apoptosis. MIC-1 expression can be induced by stress conditions such as tissue injury, malignancy and inflammation. Prostate-derived factor (PDF/MIC-1) may be related to cellular stress through its interaction with p53. The p53 tumor suppressor modulates cellular responses in various models of cell stress. Furthermore, there appears to be a requirement for functional p53 in PDF induction in these disparate models indicating that PDF may represent a novel target of p53 in response to cell stress.

This kit is intended for the quantification of macrophage inhibitory cytokine (MIC-1) in serum, plasma, tissue and cell lysates and other biological fluids where MIC-1 may be present.

Test Principle

This assay is a quantitative sandwich enzyme immunoassay. Plates are precoated with a polyclonal antibody specific for native human MIC–1 (capture antibody). The antibody-bound MIC-1 in standards and specimens binds to a polyclonal detection antibody. An HRP conjugated anti-detection signal antibody is added followed by substrate. Color development is stopped at the appropriate time and the plates are read.

格式：96孔夹心ELISA

应用：用于血清、血浆、组织和细胞裂解液和其他可能存在MIC-1的生物液体中巨噬细胞抑制细胞因子(MIC-1)的定量。

样本要求：人血清、血浆和尿液

特异性：人巨噬细胞抑制因子(MIC-1)

灵敏度：5 pg / mL

储存和稳定性：在规定温度下储存，自生产之日起1年。

Kit Contents:

Microwells precoated with anti human MIC-1 antibody: 1 X 96 wells

MIC-1 Standard (Purified MIC-1): 1 X 60 mL

Primary Antibody (100X Anti human MIC-1): 1 X 130 mL

10X Wash Buffer: 1 X 30 mL

Assay Dilution Buffer: 1 X 100 mL

TMB Substrate: 1 X 25 mL

Anti IgG-HRP Conjugate (100X): 1 X 130 mL