The ABS_BioTM Lactate dehydrogenase (LDH) generally exists in prokaryotic, fungal and eukaryotic cells. The measurement of cytoplasmic LDH activity is a well-accepted assay to quantify viable cell numbers and monitor cell proliferation. On the other hand, the leakage of cytoplasmic LDH caused by the damage of cell membrane integrity is also a good indicator of cell death and is used to estimate cytotoxicity. 

ABSBio™ ready-to-use LDH based Cell Viability/Cytotoxicity Detection Kit provides the easiest and sensitive means for cell proliferation and cytotoxicity assays, utilize resazurin to measure LDH activity either in the cytoplasm for live cells or in the medium for dead cells. The entire assay can be performed in a microtitler plate in mix-read format, measuring the fluorescence intensity at Ex/Em=530/590 nm. The reagent is no toxicity for cells, after the measurement of cell viability; the cells can be used for further experiments.

The kit is supplied with enough reagents for 500 tests in 96-well plate.

试剂盒组分:

应用:

Cytotoxicity and Proliferation assay: adherent and non-adherent cells, and certain tissues.

Detect proliferation in bacteria, yeast, fungi and protozoa as well. 

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