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Catalase 过氧化氢酶(牛肝)
货号:H0531 | 规格:1g | 价格:¥0.00 | 品牌:BMASSAY
BMASSAY® 提供Catalase 过氧化氢酶(牛肝)。BMASSAY®提供众多高纯度的生化试剂,包括抗生素、氨基酸、维生素、酶和辅酶、蛋白质、缓冲液、染色剂、层析介质、动植物激素、糖 碳水化合物、酸和盐、碱基 核酸及其衍生物等。
过氧化氢酶,Catalase from bovine liver,又称为:H2O2:H2O2 oxidoreductase; H2O2:H2O2氧化还原酶,CAS号 9001-05-2。

mol wt: ~250 kDa(tetramer);
Activity: 2,000-5,000 units/mg protein;
Isoelectric point:5.4;
Suitability: suitable for cell culture;
Storage: −20°C。过氧化氢酶的溶液不宜冷冻。

Components
Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Application
Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalses have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Catalase from bovine liver is used for the following applications:
• Gel filtration
• Analytical filtration on Ultrogel and glycerol gradient centrifugation
• Size exclusion chromatography (HPLC)-HPLC analysis of estrogen receptor
• Enzyme assays
• Alcohol oxidase activity

Biochem/physiol Actions
This product doesn not need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate.

激活过氧化氢的分解,用于研究活性氧在基因表达和细胞凋亡中的作用的天然抗氧化剂。
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