胶原酶，又称：梭菌肽酶A，Collagenase，来源于溶组织梭菌，Collagenase from Clostridium histolyticum，CAS:9001-12-1。

I型胶原酶从溶组织梭菌制备，用于细胞和组织的解离。

I型胶原酶可应用于如下组织制备细胞：

• 上皮组织
• 肺
• 脂肪
• 肾上腺组织

胶原酶是一种蛋白酶，它能够特异性的结合 Pro-X-Glyc-Pro序列中，中性氨基酸（X）和甘氨酸之间的肽键。该序列高频率的存在于胶原中。胶原酶是唯一的一种可以降解广泛存在于结缔组织中的具有三股螺旋的天然胶原纤维的蛋白酶。

Life Technologies’ group of collagenase products are purified from Clostridium histolyticum. They are intended for cell and tissue disaggregation. Collagenase is a protease with specificity for the bond between a neutral amino acid (X) and glycine in the sequence Pro-XGly-Pro.

This sequence is found in high frequency in collagen. Collagenase is unique among proteases in its ability to degrade the triplehelical native collagen fibrils commonly found in connective tissue. The collagenase most commonly used for tissue dissociation is a crude preparation containing clostripiopeptidase A and a number of other proteases, polysaccharidases, and lipases. This crude preparation is ideally suited for tissue dissociation because it contains the enzyme required to attack native collagen and reticular fibers, in addition to the enzymes which hydrolyze the other proteins, polysaccharides, and lipids in the extracellular matrix of connective and epithelial tissues. Crude collagenase does exhibit lot-to-lot variability and may produce occasional toxicity. The activity of these crude collagenase preparations has been correlated with their effectiveness at dissociating specific tissue types leading to the classification of crude collagenase preparations by type. These selected types have been found to give better performance in preparation of cells from the various tissues

Unit Definition
One protease unit liberates 1 µmol of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5.

Storage：2-8°C.