胶原酶，即：胶原蛋白酶，又称：梭菌肽酶A，英文名称：Collagenase，来源于溶组织梭菌，Collagenase from Clostridium histolyticum，CAS:9001-12-1, 性状:棕色或棕褐色结晶性粉末。

EINECS编号232-582-9；Enzyme Commission(EC)号：3.4.24.3；MDL编号：MFCD00130830。

胶原酶IV型，Collagenase Type IV, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid.

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of reaction varies from 15 minutes to several hours and yields satisfactory cell dissociation without causing too much cell death. Krebs Ringer buffer with calcium and BSA is preferred and Zn2+ is required for activity.

应用
Collagenase has been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products (AGE). The enzyme has also been used along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues. It is also effective in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes. This enzyme is tested for suitability for the release of hepatocytes, at approx. 1 mg/mL conc., in a total volume of 100 mL for each rat liver.

Storage：−20°C.