Block Ace由牛奶蛋白制备而成，是一种高性能的新型免疫实验阻断剂/封闭剂。由于乳蛋白中包含许多疏水基团，因此它对塑料和膜过滤器具有很高的亲和力。因此，与传统的阻断剂如牛血清白蛋白(BSA)相比，Block Ace阻断剂适合在进行酶联免疫吸附试验(ELISA),放射免疫测定(RIA)和免疫印迹(Immunoblotting)时阻断抗原或抗体的非特异性结合。
关键特性
● 由于该产品具有高阻断作用，并将背景维持在低水平，因此可用于ELISA等低浓度检测。
● 经过热处理，牛源抗体、酶和病毒被灭活；
● 在加热和冷冻时是稳定的；
● 能稳定生理活性物质，可作为稀释剂使用；
● 与BSA溶液相比，Block Ace更经济；
● 能在水中迅速溶解。
使用方法
Four (4) grams of Block Ace powder is dissolved into 100 mL of purified water, and this solution is used as a concentrate.
Blocking:
For blocking purpose, either concentrate or 2-4× diluted solution with purified water is used (4× diluted solution is used for ELISA; concentrate is used for Western Blotting).
Dilution:
For dilution of samples and labelled antibody, it is diluted to 10× with purified water.
Washing:
For washing operation such as B/F separation, it is diluted to 10× with purified water, and the required volume of Tween 20 (0.05%–0.2%) is added.
运输方法
Room temperature (it is cold transported)
(This product is heat treated, but there is no preservative or antibiotic. Once dissolved, it should be frozen before storage)
Block Ace与1％BSA溶液之间的封闭效果比较
Anti-human kappa chain antibody and anti-human ramda chain are coated, and it is blocked with either Block Ace or 1% BSA. Human IgG ELISA demonstrates that Block Ace has low background and a steep standard curve.
Block Ace: Blocking reagent还提供其他规格: UK-B80, 80g; UK-B40, 40g
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