The LudgerSep LS-N buffer was developed for the preparation of the LudgerSep normal phase buffer (50 mM ammonium formate buffer, pH 4.4) used in amide or HILIC (hydrophilic interaction liquid chromatography) HPLC analysis of 2-AB and 2-AA labeled glycans.

Includes 50ml of x40 LS-N buffer (2.0M ammonium formate buffer/solution) in a square HDPE bottle with leak-proof cap.

Dilute with de-gassed HPLC grade water (use 1 part of x40 buffer to 39 parts of water) to make the buffer (50 mM ammonium formate, pH 4.4). The 50 ml of x40 buffer will make 2 litres of LS-N buffer.

Use the buffer for glycan analysis with any of the following HPLC columns:
• LudgerSep N series columns
• Tosoh Amide-80 columns
• Waters ACQUITY UPLC BEH glycan columns
• GlycoSep N and N-Plus columns<
• Other HILIC HPLC columns for glycan analysis

Storage Store unopened bottles of N buffer below 25 ˚C. As with any HPLC solvent we recommend preparation of the solvent immediately before use. Take care that the undiluted/diluted solvent is not exposed to excessive heat or sunlight as it contains volatile components. Stability times of the prepared solvent will vary according to your laboratory conditions. Longterm storage of the prepared solvent, ie longer than 1 month, at room temperature, may result in evaporation of some of the volatile components in the solvent leading to a change in retention times.