Neutralization of ATIII抗体对,  中和抗凝血酶抗体对

Matched-Pair Antibody Set For Neutralization of human Antithrombin (ATIII) in Plasma

Affinity Biologicals Neutralization of ATIII Paired Antibody Set抗体对(配对抗体)包括中和抗体和阴性对照。

Affinity’s Neutralization of ATIII paired antibody set consists of Neutralizing Antibody and Negative Control. The Principle of ATIII (Antithrombin III) Neutralization – A polyclonal sheep antibody to human ATIII is added directly to human plasma and allowed to incubate for 20 minutes. During this time the antibody will bind to and inactivate (neutralize) the ability of ATIII to inhibit thrombin or activated factor X, even in the presence of heparin. Clot-based tests can then be performed on the neutralized sample. To control for the addition of antibody volume and buffer, a control antibody (non-immune sheep IgG, at the same concentration and in the same buffer) is added separately to a duplicate sample and the test is repeated.

Supplied Materials:

Neutralizing IgG (AT-NU10-T): One purple-capped vial containing 0.1 mL of sheep antibody (IgG) to human ATIII.

Control IgG (AT-NU10-C): One white-capped tube containing 0.1 mL of sheep IgG prepared from non-immune serum for use as a negative control.

Storing: between 2°C and 8°C

Type: Liquid

Antithrombin (ATIII):

Antithrombin, 又称抗凝血酶III,Antithrombin III (ATIII),是SERPIN蛋白酶抑制剂家族的一员，是血浆中主要的凝血酶抑制剂。在肝脏中生成，其血浆浓度约为200μg/ml (3.5μM)。与抗凝血酶抑制剂有关的丝氨酸蛋白酶包括凝血酶，凝血因子X、IX、XI和XII的活性形式，以及激肽释放酶、纤溶酶和尿激酶。抗凝血酶的抑制作用是通过溶蛋白性在Arg885-Ser886上裂解，随后迅速形成稳定的、非活化的1:1酶-抗凝血酶复合物。肝素能加强一些抗凝血酶的活性抑制作用。比如，最适浓度的肝素能将凝血酶和活化的凝血因子X的抑制率放大1000倍。然而，肝素对凝血因子XI、凝血因子XII和激肽释放酶的抑制率的影响却很小。抗凝血酶是一个分子量为59 kDa的单链分子，当与凝血酶作用时会形成分子量为96 kDa 的稳定的SDS型凝血酶-抗凝血酶复合物。

Paired Antibody Set, Antibody pair

配对抗体(抗体对)是指可以同时结合在一个抗原分子的不同抗原决定簇的两个抗体, 一对配对抗体对包括捕获抗体和检测抗体。

Affinity Biologicals是全球血栓形成和止血研究及诊断产品的主要生产商,产品包括凝血和止血相关蛋白抗体,偶联物,用于人和动物免疫分析的抗体对(Antibody Pairs),VisuLize系列凝血因子抗原ELISA试剂盒。

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