PGE2 Multi-Format ELISA试剂盒

DetectX®Prostaglandin E2 (PGE2) Multi-Format ELISA试剂盒是一个快速,高灵敏,低样本量的试剂盒,用于定量检测血清,血浆,尿液,组织,唾液和培养基样品中存在的PGE2。

检测类型:Competitive ELISA

样本类型:Serum, Plasma (EDTA and Heparin), Saliva, Urine, Tissue Culture Media

灵敏度:3.07 pg/mL

适应物种:PGE2在不同物种间是相同的

检测时间:2.5小时 or Overnight

样品数/板:38 in Duplicate

读数:Colorimetric, 450 nm

多格式检测:

DetectX®PGE2 ELISA试剂盒仅需一个试剂盒即可测定所有样品，该单克隆抗体具有超快的动力学，并在2或16小时孵育后显示出相同的结合特性。试剂盒可在不到3小时的时间内测量低至2 pg/mL的样品；它具有极好的交叉反应性和低样品要求。

PGE2 ELISA试剂盒提供了PGE2标准品以生成测定的标准曲线，从标准曲线中读取所有样品。将标准品或稀释的样品吸移到包被有抗体的微孔板中以捕获小鼠抗体。将PGE2-过氧化物酶偶联物添加到孔中，通过添加PGE2单克隆抗体来启动结合反应。在任一孵育后，洗涤板并添加底物，底物与结合的PGE2-过氧化物酶缀合物反应。孵育30分钟后，在450nm处读取产生的颜色的强度。在进行操作前请阅读完整试剂盒说明书或咨询中昊新生。

The PGE2 Multi-Format ELISA Kit quantitatively measures PGE2 in serum, plasma (EDTA and Heparin), saliva, urine, and tissue culture media. The PGE2 Multi-Format ELISA Kit is a competitive ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.

Use our provided PGE2 standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-mouse IgG antibody. Add the Estradiol peroxidase conjugate and the Estradiol monoclonal mouse antibody. Then incubate the mixture covered at room temperature, shaking for 2 hours. The immunological reaction occurs between the anti-PGE2 monoclonal antibody, the PGE2 antigen in the sample or standard, and the PGE2-peroxidase conjugate. As the PGE2 concentration in the sample increases, the bound PGE2-peroxidase conjugate decreases, causing a decrease in signal and vice versa. 

After the 2-hour incubation, wash away the excess PGE2-peroxidase and add the TMB substrate. The TMB substrate reacts with the bound PGE2-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the PGE2 concentration in the samples.

背景:

Eicosanoid signal transduction pathways are highly conserved and involved in several physiological processes. Prostaglandins are synthesized from arachidonic acid by cyclooxygenase (COX)-1 or -2, which converts the acid into PGH2. Cytosolic or microsomal prostaglandin synthases further process PGH2 to become PGE2 or several other prostanoids. Prostacyclin is the primary cyclooxygenase product in blood vessel walls, and it is present in inflammatory fluids in similar concentrations to PGE2. Prostacyclin is a potent vasodilator and is more powerful than PGE2 in producing hyperalgesia. Various tissues have PGE2 and several pathological conditions, including inflammation, arthritis, fever, tissue injury, endometriosis, and multiple cancers. Other biological actions of PGE2 include; vasodilation, modulation of sleep/wake cycles, and facilitation of human immunodeficiency virus replication. It elevates cAMP levels, stimulates bone resorption, and has thermoregulatory effects. It is a regulator of sodium excretion and renal hemodynamics.

Arbor Assays为临床重要生物分子研发最高质量的检测和免疫分析试剂盒,在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。Arbor Assays检测试剂盒具有高灵敏性,适用多物种及操作步骤简单的特点。

北京中昊新生科技作为美国Arbor Assays中国代理,一直为国内客户提供及时高效的配送和更专业的产品技术支持。为您提供PGE2 Multi-Format ELISA试剂盒最新现货优惠信息。