Epitope tags provide a convenient way to isolate interacting proteins without the need for specific antibodies to each new protein. His-tag is a short amino acid sequence consists of histidine (His) residues in recombinant proteins.

ABSBio™ His-tag Protein one-step Assay Kit provide a convenient, ready-to-use, high-throughput platform for rapid capture and detection of His-tagged proteins in different samples. The High Sensitivity plate is coated with the His-tagged protein and pre-blocked to provide timesaving for high-throughput users. Series dilutions of His-protein standards (28kD) and samples are added to each test well, then, anti-His tag mAb complex is added to the plate. His-tagged protein in solution and pre-coated His-tagged protein compete to bind the antibody complex. After washing steps, TMB substrates is added, incubated for detection, and a blue color is developed. Reaction is stopped and color turns to yellow when Stopping Solution is added. The yellow color intensity proportionally correlates to the concentration of the His-tagged protein standard and His-tagged protein in samples.

ABSbio’s kit is a competitive assay that generates a reverse curve, with the lowest OD values obtained from high His concentrations, and allows you to detect and quantify His-tagged protein samples simply and reliably by comparing your unknown samples to a known recombinant standard. The kit can be used to detect N-terminal and C-terminal His-tagged proteins. This kit contains all reagents required for His-tag protein detection, suitable for most samples, such as bacterial, yeast, mammalian cell lysates and cell culture supernatants, and the test completed within 1 hours. The kit can detect as little as 1 ng/mL His-tagged protein in samples.

试剂盒组分

储存及处理
Shipping on ice. Store Standard & Detection Ab at -20°C, other components at 2-8°C. Shelf life: 3 months after receipt. Warm Reagents to room temperature before use.

应用:Measurement of His fusion protein in serum, plasma, culture media and cell lysate.

反应种属:Human, Mouse, Rat

样品类型:serum, plasma, cell and tissue culture supernatant, and homogenate cell and tissue samples

检测方法:Colorimetric Sandwish ELISA assay (OD450 nm)

检测时间:1.5 hrs

储存:-20°C & 2-8°C

保质期:3 months after receipt.

运输:Icepacks

所需其他材料:
Multi-channel pipette for washing

Cell culture incubator

Orbital shaker

micro plate reader