人表皮生长因子ELISA试剂盒

ABSbio™ 人EGF ELISA试剂盒用于定量检测天然或重组人EGF。试剂盒提供的试剂足以满足96孔ELISA板中检测hEGF。

适用样品：血清，血浆和细胞培养基

检测范围：5-1000 pg/ml

Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR.

ABSbio™ Human EGF ELISA Kit provide a rapid, sensitive and reliable assay for the determination of Human EGF in serum, plasma & cell culture media. The strip plate is coated with the antibody and pre-blocked to provide timesaving for high-throughput users. Human EGF containing samples or standards and a biotinylated detection antibody directed against Human EGF are added subsequently and then followed by washing step. Streptavidin-HRP was added and unbound conjugates were washed away. Finally, TMB was used to visualize HRP enzyme reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EGF in samples.

The kit is designed to quantitatively measure natural and/or recombinant Human EGF within the range of 5–1000 pg/ml. The kit is sufficient to assay human EGF in approximately 96 ELISA plate wells.

试剂盒组分


别名：URG; Epidermal Growth Factor; Beta-Urogastrone; Pro-epidermal growth factor