人GM-CSF ELISA试剂盒用于定量检测天然或重组人GM-CSF。

ABSbio™ Human GM-CSF ELISA Kit是一种快速、灵敏、可靠的检测方法，试剂盒提供的试剂足以在96孔ELISA板中检测GM-CSF。

适用样品：血清，血浆和细胞培养基；

检测范围：8-1000 pg/ml；

别名：CSF2; GMCSF; Sargramostim; Molgramostin; Granulocyte-Macrophage Colony Stimulating Factor; Colony Stimulating Factor 2, Granulocyte Macrophage; 粒细胞-巨噬细胞集落刺激因子

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2, is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages.

ABSbio™ Human GM-CSF ELISA Kit provide a rapid, sensitive and reliable assay for the determination of Human GM-CSF in serum, plasma & cell culture media. The strip plate is coated with the antibody and pre-blocked to provide timesaving for highthroughput users. Human GM-CSF containing samples or standards and a biotinylated detection antibody directed against Human GM-CSF are added subsequently and then followed by washing step.  treptavidin-HRP was added and unbound conjugates were washed away. Finally, TMB was used to visualize HRP enzyme reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human GM-CSF in samples.

The kit is designed to quantitatively measure natural and/or recombinant Human GMCSF within the range of 8–1000 pg/ml. The kit is  ufficient to assay human GM-CSF in approximately 96 ELISA plate wells.