ABSbio™牛BMP2 ELISA试剂盒/牛骨成型蛋白2 ELISA试剂盒,旨在以夹心ELISA形式定量检测血清,血浆和细胞培养基样品中的牛BMP2,检测范围62–4000 pg/ml, 灵敏度8 pg/ml。

This kit is for quantification of BMP2 in cow, cattle, bull. This is a shorter ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance and the sensitivity of the assays. An antibody specific for bovine BMP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP2 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for bovine BMP2 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of BMP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage:

Store the standard and detection antibody at 4°C or -20°C, 10 x reagent diluents and the antibody plate at -20°C and the rest of the kit at 4°C. The kit should be used in 3 months.