牛IL-1ra ELISA试剂盒, 牛白介素1受体拮抗剂ELISA试剂盒,旨在以夹心ELISA形式定量检测样品中的牛IL-1ra含量,检测范围35–2200 pg/ml。
This is a shorter ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for bovine IL-1ra has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1ra present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for bovine IL-1ra is added to the wells. Following wash to remove any unbound
antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of IL-1ra bound in the initial step. The color development is stopped and the intensity of the color is measured.
Storage:
Store at 4 °C. The kit can be used in 3 months.