牛TNF-α ELISA试剂盒,牛肿瘤坏死因子α ELISA试剂盒,旨在以夹心ELISA形式定量检测样品中的牛TNFa含量,检测范围37–2000 pg/ml。

This kit is for quantification of TNFα in cow, cattle and bull. This is a shorter ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotinstreptavidin chemistry to improve the performance of the assays. An antibody specific for bovine TNFα has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any

TNF-α present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for bovine TNF-α is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of TNF-α bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage:

Store at 4 °C. The kit can be used in 3 months.