Fibrinogen is a soluble glycoprotein that circulates in the blood and is converted to insoluble fibrin by thrombin in the final step of the coagulation cascade. Hepatic expression of fibrinogen increases two to four hundred fold during the acute phase response to infection or inflammation. Elevated fibrinogen levels are correlated with cardiovascular disease and atherosclerosis. The sensitive quantitative measurement of total human fibrinogen antigen in plasma and serum samples is easily performed with this 96 well strip format ELISA kit. The concentration of fibrinogen in normal human plasma ranges from 1.5 to 4.5 mg/ml. The assay measures total human fibrinogen in the 3.125-800 ng/ml range. Samples giving human fibrinogen levels above 800 ng/ml should be diluted in 1X diluent before use. A 1:100,000 to 1:1,000,000 dilution for plasma or a 1:100 dilution for serum is suggested for best results. Human fibrinogen will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled anti-human fibrinogen primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of fibrinogen in the samples. A standard calibration curve is prepared using dilutions of purified fibrinogen and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Human Fibrinogen Antigen Capture Plate, Avidin-HRP