Human Thrombin Antithrombin Complex total antigen assay ELISA ki
货号:HTATKT-COM
|
规格:1 kit
|
价格:¥0.00
|
品牌:Molecular Innovations
Human Thrombin Antithrombin Complex total antigen assay ELISA kit是由北京中昊新生科技有限公司提供,Molecular Innovations专注于凝血和溶栓试剂,包括PAI-977、tPA、尿激酶、凝血因子、玻连蛋白、纤维蛋白原、纤连蛋白、肾素原/肾素、前激肽释放酶、激肽释放酶、白蛋白、纤溶酶原、纤溶酶、抗纤溶酶、凝血酶、抗凝血酶、补体成分和免疫球蛋白。
Antithrombin III is a glycosylated plasma serine protease inhibitor that forms a stoichiometric complex with coagulation cascade enzymes. Thrombin is a two-chain vitamin K-dependent glycosylated serine protease that is activated from prothrombin in the coagulation cascade. Antithrombin III inhibits thrombin with heparin enhanced kinetics and forms a 1:1 covalent complex. The sensitive quantitative measurement of total human Thrombin Antithrombin (TAT) Complex antigen in plasma and serum samples is easily performed with this 96 well strip format ELISA kit. Free thrombin, prothrombin and antithrombin III will not be detected by this assay. In-house testing determined human TAT complex concentrations of 10 ng/ml in plasma and 52 ug/ml in serum. The assay measures total human TAT complex in the 1-1,000 ng/ml range. Samples giving human TAT complex levels above 1,000 ng/ml should be diluted in blocking buffer before use. Normal human plasma samples should be applied directly to the plate without dilution. A 1:500 to 1:1,000 dilution for normal human serum is suggested for best results. Human TAT complex in samples will bind to the anti-human thrombin capture antibody coated on the microtiter plate. After appropriate washing steps, monoclonal anti-human antithrombin primary antibody binds to TAT complex captured on the plate. Excess antibody is washed away and bound monoclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of TAT complex. Color development is proportional to the concentration of TAT complex in the samples. All reagents and standards are provided in these ELISA kits.
相关产品
-
货号:FXII5B5
|
规格:1.0 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:SASHFXII-GF-FITC
|
规格:10 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:SASMFIX
|
规格:1.0 ml
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:RTPAKT-TOT
|
规格:5 kits
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:MUPA-BIO
|
规格:0.05 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:KNG15G5
|
规格:0.1 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:H77B6
|
规格:10 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:CTNT3B10
|
规格:0.1 mg
|
价格:¥0.00
|
品牌:Molecular Innovations