Human uPA total antigen assay ELISA kit
货号:HUPAKT-TOT
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规格:5 kits
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价格:¥0.00
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品牌:Molecular Innovations
Human uPA total antigen assay ELISA kit是由北京中昊新生科技有限公司提供,Molecular Innovations专注于凝血和溶栓试剂,包括PAI-1078、tPA、尿激酶、凝血因子、玻连蛋白、纤维蛋白原、纤连蛋白、肾素原/肾素、前激肽释放酶、激肽释放酶、白蛋白、纤溶酶原、纤溶酶、抗纤溶酶、凝血酶、抗凝血酶、补体成分和免疫球蛋白。
Urokinase plasminogen activator (uPA), along with its receptor uPAR, is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion and migration. Clinical studies have indicated that high uPA levels may elevate the risk for tumor invasion and metastasis. Increased expression and activity can exert potent arthritogenic properties in rheumatoid arthritis patients. Increased uPA activity may be an implication for the pathophysiology of endometriosis. The sensitive quantitative measurement of total human uPA antigen in plasma, serum, culture media or tissue extract samples is easily performed with this 96 well strip format ELISA kit. The concentration of uPA antigen in human plasma has been reported to be 3.5 ng/ml. The assay measures total uPA in the 0.1-50 ng/ml range. Samples giving human uPA levels above 50 ng/ml should be diluted in blocking buffer before use. Human uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed uPA will be detected by the assay. After appropriate washing steps, anti-human uPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of human uPA in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified human uPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
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