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Mouse CRP ELISA Kit
货号:MCRPKT | 规格:5 kits | 价格:¥0.00 | 品牌:Molecular Innovations
Mouse CRP ELISA Kit是由北京中昊新生科技有限公司提供,Molecular Innovations专注于凝血和溶栓试剂,包括PAI-1248、tPA、尿激酶、凝血因子、玻连蛋白、纤维蛋白原、纤连蛋白、肾素原/肾素、前激肽释放酶、激肽释放酶、白蛋白、纤溶酶原、纤溶酶、抗纤溶酶、凝血酶、抗凝血酶、补体成分和免疫球蛋白。
C-reactive protein (CRP) is an acute phase reactant which is elevated in plasma in response to increased interleukin-6 induced by inflammation, infection and tissue injury. CRP is expressed mainly in the liver and activates the complement pathway following calcium-dependent binding to phosphocholine on apoptotic, necrotic and microbial cells. The sensitive quantitative measurement of total mouse CRP antigen in plasma and other biological fluids is easily performed with this 96 well strip format ELISA kit. This kit has been validated for measurement of CRP in C57 mouse plasma (20 ug/ml), Balb/C mouse plasma (3.5 ug/ml), and CD1 mouse plasma (7.2 mg/ml). The assay measures CRP antigen in the 0.02-2 ng/ml range. Samples giving mouse CRP levels above 2 ng/ml should be diluted in blocking buffer before use. For best results, dilute plasma samples 1:25,000 to 1:50,000. Mouse CRP will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-mouse CRP primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with streptavidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of CRP in the samples. A standard calibration curve is prepared using dilutions of purified CRP and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
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