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Active mouse uPA functional assay ELISA kit
货号:MUPAKT | 规格:5 kits | 价格:¥0.00 | 品牌:Molecular Innovations
Active mouse uPA functional assay ELISA kit是由北京中昊新生科技有限公司提供,Molecular Innovations专注于凝血和溶栓试剂,包括PAI-1436、tPA、尿激酶、凝血因子、玻连蛋白、纤维蛋白原、纤连蛋白、肾素原/肾素、前激肽释放酶、激肽释放酶、白蛋白、纤溶酶原、纤溶酶、抗纤溶酶、凝血酶、抗凝血酶、补体成分和免疫球蛋白。
Urokinase plasminogen activator (uPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migration. The sensitive quantitative measurement of functionally active mouse uPA in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The concentration level of uPA antigen in mouse urine has been reported to be 1.8 ug/ml. The assay measures active uPA in the 0.025-10 ng/ml range. Samples giving mouse uPA levels above 10 ng/ml should be diluted in blocking buffer before use. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1, which in turn could potentially form a complex with active uPA. If plasma samples were collected in citrate the pH should be brought up to neutral with the 10X TBS provided in the kit. Serum and cell culture media at neutral pH may also be used. If using kidney extracts that have been extracted using triton X, dialyze to remove the triton X before using in the assay. Detergents such as triton X may interfere with the assay. Functionally active uPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Inactive or complexed uPA will not bind to the PAI-1 and will not be detected by the assay. Cross-reactivity: 1percent Human uPA. After appropriate washing steps, anti-mouse uPA primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of active uPA in the samples. A standard calibration curve is prepared using dilutions of purified uPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

This assay uses an exclusive enzyme capture technology to only detect functionally active protein.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Avidin Plate, Secondary Antibody
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