Urokinase plasminogen activator (uPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migrations. The sensitive quantitative measurement of total rat uPA antigen in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The assay measures total uPA in the 0.01-10 ng/ml range. Samples giving rat uPA levels above 10 ng/ml should be diluted in blocking buffer before use. Rat uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed uPA will be detected by the assay. After appropriate washing steps, anti-rat uPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of rat uPA in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified rat uPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Suggested additional reagents: 10X Wash Buffer, TMB Substrate, uPA Antigen Capture Plate, Secondary Antibody