Pembrolizumab (Keytruda®) Pharmacokinetic ELISA Kit
Pembrolizumab ELISA Keytruda ELISA Biosimilars. The Pembrolizumab ELISA kit is designed to measure free Pembrolizumab with high specificity and sensitivity.

应用程序:Measure free Pembrolizumab
靶蛋白:keytruda
灵敏度:检测限1.5 ng/mL
检测方法:Peroxidase / OD450
精确度:Inter-assay CV <10%.
最低检出限:0.39ng/ml
储存条件:Stable at -20°C for 1 year

描述:
Pembrolizumab (Trade name Keytruda®) is a human monoclonal antibody used to block the action of Programmed Cell Death Protein 1(PD-1). Upregulation of PD-1 ligands on tumor cells can allow the tumor to evade anti-tumor immune response. By blocking PDL1 and PD-L2 binding to PD-1, Pembrolizumab induces an anti-tumor immune response by allowing full activation of the anti-tumor T cell response. Thus, Pembrolizumab acts as a checkpoint inhibitor to stimulate immune responses to eliminate cancer cells.

The Pembrolizumab ELISA kit is designed to measure free Pembrolizumab with high specificity and sensitivity. The assay design uses a pair of antibodies allowing detection of whole Pembrolizumab molecules in biological matrices.

检测原理:
This assay employs the sandwich enzyme immunoassay technique. Standards and test samples are pipetted into the appropriate wells. Pembrolizumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Pembrolizumab present in test samples.
试剂盒组分:
Coated microtiter plate, 96 wells
Calibrator diluent. - 1.8ml
Calibrator (500 ug/mL) - 12ul
10X wash buffer - 50ml
Assay buffer - 50ml
1000X detection reagent - 17ul
TMB - 12ml
TMB stop solution - 12ml
Plate sealers - 3



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