DNeasy PowerSoil HTP 96 Kit用于在不到一天的时间内从多达384个土壤样本中分离DNA.

DNeasy PowerSoil HTP 96 Kit特点
Fast, high-throughput format processes up to 250 mg of sample in 96 wells
Easy isolation of DNA from tough microbes using the PowerLyzer 24 Homogenizer
Completely eliminates PCR inhibitors with Inhibitor Removal Technology

Processing large amounts of soil and other environmental samples is fast and easy with the DNeasy PowerSoil HTP 96 Kit. Using a 96-well format, difficult soil types from compost to marine sediment to manure can be successfully processed and the DNA isolated. The highly pure DNA resulting from processing with the DNeasy PowerSoil HTP 96 Kit is ready for downstream applications, including qPCR amplification.

Samples are broken down and homogenized on the 96-well bead beating plate. Cells are lysed by both mechanical and chemical means and the DNA is captured on a 96-well silica membrane spin column, washed and eluted. The DNA is then ready for downstream applications such as qPCR or next-generation sequencing.

PCR analysis on resulting DNA has detected a variety of organisms, from yeasts, molds and other fungi, algae, Gram (+/-) bacteria (including Bacillus subtilis, Bacillus anthracis), actinomycetes (including Streptomyces) and nematodes. DNeasy PowerSoil HTP 96 Kit was formerly sold by MO BIO as PowerSoil-htp 96 Well Soil DNA Kit.

DNeasy PowerSoil HTP 96 Kit技术参数
Format:Silica Spin Filter Plates
Processing:Bead beating
Binding capacity:Up to 20 µg per prep
Sample size:0.25 g
Throughput:96-384 samples
Time per run or per prep:Process 384 samples in less than one day
Storage temperature:Store at room temperature(15-30°C)
Bead size:0.7 mm garnet
Sample types:Processed soil, fecal, water, food, insects, swabs with PCR inhibitors.