Gentra Puregene Tissue Kit For 4 g tissue: RNase A Solution, Puregene Proteinase K, and Reagents

Gentra Puregene Kits are designed for purification of high-molecular-weight genomic, mitochondrial, or viral DNA from a variety of sample sources. High-quality DNA can be purified from sample types including whole blood, buffy coat, bone marrow, body fluids, cultured cells, animal tissues, Gram-negative bacteria, Gram-positive bacteria, and yeast in as little as 25 minutes. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cations, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.

Principle and procedure
Cells are lysed with an anionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in hydration solution (1 mM EDTA, 10 mM Tris·CI pH 7.5). Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2–8°C, –20°C, or –80°C.