QIAseq FX DNA Library Kit For high-performance, all-enzymatic whole genome and hybrid capture library preparation for Illumina® instruments

Since its introduction, next-generation sequencing (NGS) has found numerous applications with considerable impact and potential, including cancer research, stem cell research, metagenomics, population genetics and biomedical research. While NGS technology is continuously improving, library preparation remains one of the main bottlenecks as it involves several time-consuming steps. Not only can library preparation result in considerable sample loss, there is also the potential to introduce handling errors.

QIAGEN QIAseq FX technology incorporates all-enzymatic DNA fragmentation into a streamlined, optimized protocol that does not require sample cleanup between fragmentation and adapter ligation. This saves time and prevents errors. Optimized enzyme and buffer compositions ensure high sequencing library yield. A simple, three-reaction protocol enables straightforward automation of library preparation on various liquid-handling platforms, reducing hands-on time and run-to-run variability.

Benefits of the QIAseq FX DNA Library Kit:
• Sequencer-ready libraries from genomic DNA in just 2.5 hours
• Fragment size, input amount and batch size customizable to any sequencing experiment
• Low G/C bias for even genomic coverage
• Superior performance compared to other enzymatic fragmentation methods
• Convenient kit format including dual-barcoded adapters

Fast, convenient, all-enzymatic library preparation

Overcome the limitations of current short-read NGS DNA fragmentation technologies. The new QIAseq FX DNA Library Kit takes you from 1 ng – 1 µg genomic DNA to sequencerready, amplified libraries in just 2.5 hours. It’s faster and easier to automate than mechanical shearing but still generates the high-quality libraries you need for whole genome sequencing of any organism.