QIAprep 2.0 Spin Miniprep Columns (100 spin columns) For purification of up to 20 μg molecular biology grade plasmid DNA.

特征
Ready-to-use plasmid DNA in minutes
Reproducible yields of molecular biology grade plasmid DNA
Single protocol for high- and low-copy vectors

The QIAprep Spin Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning.

QIAprep Spin Miniprep standard protocols can also be executed using the TRACKMAN Connected system, paired with PIPETMAN M Connected pipettes, both from Gilson.

The QIAprep Spin Miniprep Kit enables purification of up to 20 μg molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as PCR, sequencing and cloning. Versatile QIAprep spin columns can be used either in microcentrifuges, on vacuum manifolds, or in the QIAcube. The vacuum procedure provides simplified handling and faster sample processing. QIAprep spin columns can be vacuum processed using the QIAvac 24 Plus or any other commercial manifold with luer connectors. The QIAprep Spin Miniprep Kit can now also be fully automated on the QIAcube.

QIAprep spin columns contain a unique silica membrane that binds up to 20 μg DNA in the presence of a high concentration of chaotropic salt, and allows elution in a small volume of low-salt buffer. QIAprep membrane technology eliminates time consuming phenol-chloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries. High-purity plasmid DNA eluted from QIAprep spin columns is immediately ready to use - there is no need to precipitate, concentrate, or desalt.

程序
Plasmid purification using QIAprep Kits follows a simple bind-wash-elute procedure. First, bacterial cultures are lysed and the lysates are cleared by centrifugation. The cleared lysates are then applied to the QIAprep module where plasmid DNA adsorbs to the silica membrane. Impurities are washed away and pure DNA is eluted in a small volume of elution buffer or water.

In addition to plasmid purification from Escherichia coli, QIAprep Kits can be used to purify plasmid DNA from Saccharomyces cerevisiae, Bacillus subtilis, and Agrobacterium tumefaciens. Contact QIAGEN Technical Services or your local distributor for protocols for these applications.

应用
The QIAprep Miniprep Kits provides reproducible yields of high-purity DNA suitable for use in most applications, including:

PCR
Restriction digestion
Ligation and transformation
Sequencing
Screening

Compatible with Gilson's TRACKMAN Connected system
QIAGEN has programmed QIAprep Spin Miniprep protocols into the TRACKMAN Connected system, which controls the Bluetooth-enabled PIPETMAN M Connected pipettes sold by Gilson.

TRACKMAN Connected guides researchers through the QIAprep Spin Miniprep protocols while automatically adjusting pipette settings. Each step of the protocol execution is recorded to accelerate reporting, because the system generates a comprehensive run report.