QIAseq miRNA Library Auto Kit For 384 sequencing prep reactions on automation platforms. Includes at least 50% more volume of reagents for: 3' ligation, 5' ligation, reverse transcription, cDNA cleanup, library amplification and library cleanup reagents; quality control primers

Precision small RNA library kit for Illumina® NGS systems using automated library preparation.

QIAseq miRNA enables Sample to Insight®, precision next-generation sequencing (NGS) of mature miRNAs on Illumina NGS instruments. This highly optimized solution facilitates both enhanced differential expression analysis using integrated unique molecular indices (UMIs) and novel discovery of miRNA from cells, tissues, and biofluids. The required amount of template for a single QIAseq miRNA sequencing reaction can range from 500 ng to as little as 1 ng of purified total RNA.

In recent years, NGS has emerged as a highly advanced research tool for both high-throughput miRNA expression analysis and novel miRNA discovery. Among commercially available solutions, QIAseq miRNA defines a new generation of small RNA sequencing products and includes several distinct features not found in other sequencing kits. The standard QIAseq miRNA procedure does not require gel purification, excision, and elution, which substantially reduces the required hands-on time and noticeably shortens the length of the whole workflow.

Proprietary methodology utilizing modified oligonucleotides efficiently prevents adapter– dimerization in the sequencing library and the highly optimized reaction chemistry virtually eliminates biases and background contaminants, facilitating the preparation of robust, miRNAspecific libraries. The kit also integrates UMIs into the reverse transcription process, enabling unbiased and accurate miRNome-wide quantification of mature miRNAs by NGS. Should a library fail presequencing quality control (QC), in-line controls are included in the library generation procedure to allow the use of real-time PCR for fast and efficient troubleshooting.

Both primary and secondary data analysis solutions have been developed to facilitate rapid and robust UMI counting, miRNA mapping, and differential expression analysis. Overall, QIAseq miRNA offers an unrivaled Sample to Insight solution for differential expression analysis and discovery of novel miRNAs using NGS.